Replication Slippage of Different DNA Polymerases Is Inversely Related to Their Strand Displacement Efficiency
نویسندگان
چکیده
منابع مشابه
Mechanism of strand displacement synthesis by DNA replicative polymerases
Replicative holoenzymes exhibit rapid and processive primer extension DNA synthesis, but inefficient strand displacement DNA synthesis. We investigated the bacteriophage T4 and T7 holoenzymes primer extension activity and strand displacement activity on a DNA hairpin substrate manipulated by a magnetic trap. Holoenzyme primer extension activity is moderately hindered by the applied force. In co...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1999
ISSN: 0021-9258
DOI: 10.1074/jbc.274.39.27481